Table 5.
Association of unique structural protein mutations identified in RGCB isolates with potential T-cell epitopes
| Sl. no. | Viral protein | Position in the polypeptide | Mutation | Associated HLA allelesa | Isolates that show the presence of the mutation | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| RGCB03/2006 | RGCB05/2006 | RGCB80/2007 | RGCB120/2007 | RGCB355/2008 | RGCB356/2008 | |||||
| 1 | Capsid | 28 | I → T | HLA-B*27, HLA-B*51, HLA-B*53 | – | – | – | – | – | Y |
| 2 | 199 | G → D | HLA-A*0301, HLA-A*1101, HLA-A*24, HLA-A*6801, HLA-B*07, HLA-B*27 | – | Y | – | – | – | – | |
| 3 | E3 | 305 | R → G | HLA-A*0101, HLA-A*0201, HLA-A*0202, HLA-A*0203, HLA-B*27 | – | – | – | – | – | Y |
| 4 | E2 | 554 | V → I | HLA-A*0101, HLA-A*0206, HLA-A*0301, HLA-A*1101, HLA-A*6802 | – | – | – | – | – | Y |
| 5 | 577 | K → Q | HLA-A*0301, HLA-A*1101, HLA-B*40, HLA-B*44 | – | – | Y | Y | Y | – | |
| 6 | 641 | E → K | HLA-A*0201, HLA-A*0202, HLA-A*6802, HLA-B*40, HLA-B*44 | Y | – | – | – | – | – | |
| 7 | 675 | G → S | HLA-B*40, HLA-B*44, HLA-B*51 | – | – | – | – | – | Y | |
| 8 | 686 | E → G | HLA-A*0101, HLA-A*0201, HLA-A*0203, HLA-A*0301, HLA-A*24, HLA-B*40, HLA-B*44 | – | – | – | Y | – | – | |
| 9 | 741 | C → G | HLA-A*0201, HLA-A*0202, HLA-A*0203, HLA-A*0206, HLA-A*0301, HLA-A*24, HLA-A*3101, HLA-A*6802 | – | – | – | Y | – | – | |
| 10 | 6K | 808 | S → G | HLA-A*0101, HLA-A*0201, HLA- A*0202, HLA-A*0206, HLA-B*51 | – | – | – | Y | – | – |
| 11 | E1 | 813 | V → A | HLA-A*0201, HLA-A*0202, HLA-A*0206, HLA-A*24, HLA-A*6802, HLA-B*51 | – | – | – | – | – | Y |
| 12 | 926 | E → G | HLA-A*0301, HLA-A*1101, HLA-A*6801, HLA-B*40, HLA-B*44 | Y | – | – | – | – | – | |
| 13 | 983 | D → G | HLA-A*0202, HLA-A*0203, HLA-A*0206, HLA-A*0301, HLA-A*1101, HLA-A*24, HLA-A*6801, HLA-A*6802, HLA-B*51 | – | – | – | Y | – | – | |
| 14 | 994 | Y → H | HLA-A*0101, HLA-A*24, HLA-B*07 | Y | – | – | – | – | – | |
| 15 | 1035 | A → V | HLA-A*0101, HLA-A*0201, HLA-A*0202, HLA-A*0203, HLA-A*0206, HLA-A*0301, HLA-B*07, HLA-B*27, HLA-B*51 | – | – | Y | Y | Y | Y | |
| 16 | 1057 | G → E | HLA-A*0201, HLA-A*0202, HLA-A*0203, HLA-A*24 | – | – | – | – | Y | – | |
| 17 | 1205 | T → A | HLA-A*0201, HLA-A*0203, HLA-A*0206, HLA-A*24, HLA-B*51 | Y | – | – | – | – | – | |
| 18 | 1220 | K → R | HLA-A*0202, HLA-A*0206, HLA-A*0301, HLA-A*1101, HLA-A*6802, HLA-B*51 | – | – | – | – | Y | – | |
aPredicted epitopes are given in Supplementary Tables 2 and 3. Y Indicates presence of mutation