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Removal of GroEL by urea. SDS-PAGE analysis of GroEL-bound S1c protein domain treated by urea. The results suggest that GroEL was removed when GST-S1c was washed by urea at a concentration higher than 1.75 M. Lane M: prestained protein markers; lanes 1–9: GroEL-bound S1c protein domain treated with different concentrations of urea; lane 1: 0 M; lane 2: 0.5 M; lane 3: 0.75 M; lane 4: 1 M; lane 4: 1.25 M; lane 6: 1.5 M; lane 7: 1.75 M; lane 8: 2 M; lane 9: 2.5 M
