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Expression of S1c domain in P. pastoris. SDS-PAGE analysis of expression of (a) S1c and (b) S1b, as well as western blotting analysis of expression of (c) S2a and S2c of protein domains in P. pastoris. Lane M: prestained protein markers; Numbers of subsequent lanes: the number of hours after induction; lane T: total lysate; lane S: soluble fraction; lane E: eluent
