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Interaction between S1c and ACE2. Pull-down assay of S1c protein domain expressed by in P. pastoris. Western blot analysis using anti-hACE2 antibody was used to detect ACE2. Lane M: prestained protein markers; lane 1: purified S1c protein domain bound to His-bind resin was used as bait to incubate with protein lysate of VeroE6 cells; lane 2: His-bind resin in the absence of S1c was used as bait to incubate with protein lysate of VeroE6 cells
