Fig. 8.
End-point dilution ELISA analysis of the anti-TGEV antibody in murine intestinal lavage or serum after immunization: a intestinal IgA and b serum IgG. Five-week-old BALB/c mice were inoculated intragastrically with SL7207 (pVAX-M) three times in 2-week intervals at a dosage of 109 CFU per mouse. Control mice received 109 CFU of SL7207 (pVAX), while PBS group mice received PBS as a negative control. Murine intestinal lavage and serum, collected at weeks 0, 2, 4, 6, and 8 post-immunization, were analyzed by end-point dilution ELISA assay using TGEV as a coating antigen. Absorbance was measured at 450 nm in an automated plate reader. Results are expressed as means of the OD 450 ± SD (n = 3). Numbers on x-axis indicate weeks post-immunization. ** stands for P < 0.01, * stands for P < 0.05