Table 1.

Sequences of primers used to amplify CPV-2

Primer designation	Primer sequence (5′-3′)	Position	Fragment size in bp
NS1-F1	ACCGTTACTGACATTCGCTTC	781–802	2111
NS1-R1	CCTTACCTCTCCTGGCTC	2875–2891
VP2-F2	GCCGGTGCAGGACAAGTA	3317–3335	2011
VP2-R2	CAACCCACACCATAACAACA	5038–5327
CoF13	TTTATGTTTTATTACAATTTATTTTAAGATTAG	1–33	5783
CoR13	CAAACCAACCAAACCACCCA	5765–5783

These primers were the same set used in Calderon et al. [13], Chaturvedi et al. [14], and Pratelli et al. [15]