Fig. 1.

Effect of shRNAs on PEDV-induced CPE in Vero cells. Vero cells were transfected with different plasmids and then infected with PEDV at 100 TCID₅₀. CPE were examined 48 h post-infection, and cell images were captured. From (a) to (e), cells were transfected with pSilener4.1-M1 (a), pSilener4.1-N (b), pSilener4.1-S2 (c), pSilener4.1-M2 (d), and pSilener4.1-S1 (e), respectively. f, g, and h also served as transfected with pSilener4.1-NC (f), mock control (g), and normal Vero (h) in the experiment, respectively. The experiment was performed in triplicate and repeated three times