Table 2.

The inserted sequences in shRNA-expressing plasmids. List of the siRNA target sequences

Plasmids	Inserts
pSilencer4.1-M1	5′-gatccCTGGAATTTCACATGGAATTTCAAGAGAATTCCATGTGAAATTCCAGTTTTTTA-3′
pSilencer4.1-M2	5′-gatccCGTACAGGTAAGTCAATTATTCAAGAGATAATTGACTTACCTGTACGTTTTTTA-3
pSilencer4.1-N	5′-gatccCCTAAGAAGAACAAATCCATTCAAGAGATGGATTTGTTCTTCTTAGGTTTTTTA-3′
pSilencer4.1-S1	5′-gatccCGGCATAACATGGGATAATTTCAAGAGAATTATCCCATGTTATGCCGTTTTTTA-3′
pSilencer4.1-S2	5′-gatccCTGCATATGTTAATGATGATTCAAGAGATCATCATTAACATATGCAGTTTTTTA-3′
pSilencer4.1-NC	5′-gatccCTAGTATGTGCGTGCGGTTTTCAAGAGAAACCGCACGCACATACTAGTTTTTTA-3′

The first five lowercase nucleotides represent a BamHI site (gatcc) for ligation into the pSilencer4.1-CMV vectors; the subsequent uppercase oligonucleotides represent the 19 nt sense and antisense sequences specific to the mRNA target; the italics section represents a 9 nt loop sequence common to all of the hairpins, and polythymidine tracts involved in the termination of transcription are underlined (TTTTTT)