Fig. 2.

N cannot inhibit IFN-β promoter activation induced by overexpressing signaling molecules in innate immune pathways. 293T cells (1 × 10⁵) were co-transfected with Flag-N or empty vector, pIFN-β-luc, pRL-TK and indicated plasmid expressing RIG-I-2CARD (a), MAVS, TRIF (b), TBK1, or IKKε (c). At 24 h post-transfection, reporter assays were performed. The data represent the mean ± S.D. of three independent experiments. * P < 0.05