Fig. 6.

N does not interact with RIG-I or MDA5. a 293T cells (2 × 10⁶) were co-transfected with indicated expression plasmids (8 μg each). Cell lysates were immunoprecipitated with control rabbit IgG or rabbit anti-Flag (αFlag) antibody as indicated. The immunoprecipitates were analyzed by Western blots with mouse anti-Myc antibody (top panels) and mouse anti-FLAG antibody (middle panels). The expression levels of transfected proteins in the lysates were analyzed by Western blots with indicated antibodies (bottom panel). The experiments were repeated for three times with similar results. b 293T cells (2 × 10⁶) were co-transfected with indicated expression plasmids (8 μg each). At 24 h post-transfection, cells were transfected with poly (I:C) (left panels) or infected with Sendai virus (right panels) for 6 h. Then, co-immunoprecipitation assays were performed similarly as in (a)