Fig. 7.

Reporter assays using truncated N expression constructs. a Truncated constructs of N. The open reading frame of N was truncated in six constructs corresponding to the structural domains. b Western blot analysis was performed to determine the expression of SARS-CoV N protein and N truncation mutants. c The truncated N plasmids, pIFN-β-luc, pRL-TK were co-transfected into 293T cells (1 × 10⁵) for 24 h before SenV infection. Reporter assays were performed 18 h after infection. The data represent the mean ± S.D. of three independent experiments. * P < 0.05. Meanwhile, cell extracts were prepared, and the expression of β-actin protein was determined by Western blot as the expression control