Table 2.
Primers used in this study
| Primers | Location | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| 1 | 218-1526 | CATCCGTTGCTTGGGCTACCTAG | GCAGACCAACCTTCTGGTTCC |
| 2 | 1439-3082 | CACAAGTTGTTGTTCGTGGC | GAGCGTCCTTATGAACTATAC |
| 3 | 2545-3801 | GCGGGTGGCAAGACTGTCAC | AATGCCTTTGACATAAGAAGG |
| 4 | 3738-5606 | GGACTATGTTAAGAAACATGG | CCATCTCCTACTTTATCGGC |
| 5 | 5434-7340 | GTTGATGGTGTAACTATGGGC | GTACCACTAACGATACCACC |
| 6 | 7219-9220 | TCAGCGACTGTCAAGTCAGG | TACCACCATAAGAGCAAGC |
| 7 | 9171-10949 | GAAAGCAAATTGTGGTGATAG | GAGGGAATGTGTGAAAACTC |
| 8 | 10815-12586 | GGACAATTTGTTGGGTATGC | GTTCATAATTACTAGGAGTGG |
| 9 | 12412-14028 | CCTGATGTTGTAGAGCGAGCC | AAGAATGGACACACCTGCCAC |
| 10 | 13780-15652 | TTTGAATGTTATGAAGGTGG | TAGTCTTACCAGGTTCCCACG |
| 11 | 15520-16868 | TCATTAAGACGCTTTGCTG | ATGACTACAAGTATACCACGC |
| 12 | 16738-19004 | GTAGACTCTTCACAAGGTTC | TAAACATACAGATTCGCTCC |
| 13 | 18402-20306 | GGCTTCTTATAATGCAGCTG | AAATTCACAACTGGTGTTGC |
| 14 | 20070-22284 | CAAGATTGTGCATGGTGGAC | TATGTTATCACAAACAGGACC |
| 15 | 21262-22963 | ACACAAACAGCTCAGAGTGG | CTTGAATTGCATCAAGTTGC |
| 16 | 22788-24887 | CATTGGTCATATGCAGGAAGG | GGAGTATTGAACCTACGGCATTA |
| 17 | 24627-27112 | TGGATACGCAACTAGGAGTCG | CATCATCAAATTCCAGCTGTGC |
| 5′ regiona | 1-449 | CATGGCTACATGCTGACAGCCTA | TACAAACGTCGTATAGCCGACC |
| 3′ regionb | 26942-27669 | GTAAGACCAAAATCACGCCCAAG | AAGCAGTGGTATCAACGCAGAGT |
| 3′ RTc | poly-A | AAGCAGTGGTATCAACGCAGAGTAC(T)30 |
aPCR Primers used in 5′ RACE
bPCR Primers used in 3′ RACE
cRT Primer used in 5′ RACE