Figure 1.

Effects of RLX (0.5 mg/kg/day), CGP (1.44 mg/kg/day), CAND (2 mg/kg/day) or RLX with CAND or CGP on (A) systolic blood pressure measured by tail cuff and (B) ventricular weight (VW) to body weight (BW) ratio in FVB/N mice fed a high salt (HS, 5% NaCl) diet for 8 weeks. (C) Left ventricular expression of RXFP1, AT_1aR, and AT₂R mRNA levels from NS- and HS-treated mice; shown in the comparative cycle of threshold fluorescence at which RXFP1, AT_1aR, and AT₂R mRNA expression was detected, relative to the internal housekeeping gene GAPDH, by real-time PCR analysis. All treatments were given for 4 weeks, between weeks 5–8 of the HS diet. At the same time, a group of mice were fed a normal salt (NS, 0.5% NaCl) diet for comparison. All data are expressed as mean ± s.e.m. (n = 6–8 per group): (#) P < 0.05 vs HS for systolic blood pressure at 8 weeks (one-way ANOVA with Tukey correction for multiple comparisons); (∗) P < 0.05 for systolic blood pressure-time interaction compared with HS group (2-way repeated measures ANOVA).