(A) Representative images for interstitial fibrosis
stained with
picrosirius red (indicated by red) in left ventricle (LV) sections
obtained from FVB/N mice fed on a normal salt (NS, 0.5% NaCl) diet
or mice fed on a high salt (HS, 5% NaCl) diet in the presence or absence
of RLX (0.5 mg/kg/day), CGP (1.44 mg/kg/day), CAND (2 mg/kg/day),
or RLX with CAND or CGP. Scale bar for all images = 50 μm. (B)
Group data of % area of interstitial fibrosis in LV sections, or (C)
total tissue collagen measured by hydroxyproline assay in LV tissue
from same animal groups in which picrosirius red staining was performed
(n = 7–8 per group). All data are expressed
as mean ± s.e.m. (∗∗) P < 0.01, (∗∗∗) P < 0.001 vs NS; (#) P < 0.05, (##) P < 0.01, (###) P < 0.001 vs HS,
determined by One-Way ANOVA with Tukey correction for multiple comparisons.