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. 2019 Apr 4;2(3):168–182. doi: 10.1021/acsptsci.9b00001

Figure 1.

Figure 1

Abrogation of S phase arrest by CHK1i or WEE1i is insensitive to inhibition of CDK1/2 but requires CDC7 and CDC25 activity. (a) MDA-MB-231 cells were incubated with SN38 for 24 h and harvested as indicated. Cells were stained for DNA with propidium iodide and analyzed by flow cytometry. (b) Following removal of SN38 at 24 h, cells were incubated with 1 μM MK-8776 (CHK1i) or 1 μM AZD1775 (WEE1i) concurrently with 0–10 μM CVT-313 (CDK1/2i). (c) Same as panel b, except 0–10 μM XL413 (CDC7i) was added concurrently with CHKi or WEE1i. (d) MDA-MB-231 cells were incubated with SN38 for 0–24 h, and then CHK1i or WEE1i were added from 24 to 30 h, concurrently with 0–20 μM NSC663284 (CDC25i). Cells were immunostained with a fluorescent anti-pHH3 antibody, and DNA was stained with propidium iodide and then analyzed by flow cytometry. The gates and percentages shown represent the proportion of cells positive for pHH3.