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. 2019 Apr 4;2(3):168–182. doi: 10.1021/acsptsci.9b00001

Figure 2.

Figure 2

CDC25i and CDK1/2i, but not CDC7i, prevent pHH3 and γH2AX from SN38 with CHK1i or WEE1i. (a) MDA-MB-231 cells were incubated with SN38 for 24 h and then CHK1i or WEE1i from 24 to 30 h. Additionally, CDC25i, CDK1/2i, or CDC7i was administered concurrently with CHK1i or WEE1i as indicated. Whole cell extracts were probed for the indicated proteins by Western blotting. Black arrows indicate bands relatively resistant to CVT-313, and gray arrows indicate bands very sensitive to CVT-313; these arrows highlight the same bands noted in Figure 4b. (b) MDA-MB-231 cells were incubated with SN38 for 24 h. Cyclin E protein was immunoprecipitated, and the indicated proteins were probed by Western blotting.