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Impact of the concentration of CDK1/2i and CDC7i in undamaged cells. AsPC-1 cells were synchronized by incubation with nocodazole for 16 h. Mitotic cells were collected and replated in fresh medium for 4 h (left) or 12 h (right). CDK1/2i or CDC7i were administered from 4 to 16 h (left) or from 12 to 24 h (right) at the indicated concentrations. DNA was stained with propidium iodide and then analyzed by flow cytometry.
