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. 2018 Oct 12;1(2):84–95. doi: 10.1021/acsptsci.8b00007

Figure 4.

Figure 4

Aglycone polyether J1-001-2 inhibits MDA-MB-231 cell proliferation through the Wnt signaling pathway and by promoting cell autophagy. (A and B) Proteome analysis of (A) the Wnt signaling pathway and (B) autophagy and apoptosis after J1-001-2 treatment of MDA-MB-231 cells. The dashed red line means no changes (ratio = 1) between treated cells and untreated cells. (See the Supporting Information for experimental details.) (C–F) Western blot analysis of MDA-MB-231 cells treated with J1-001-2. (C and D) Cells were exposed to J1-001-2 for 16 h, and blots were probed for (C) Wnt signaling pathway proteins [Wnt, P-LRP6 (S1490), β-catenin, and P-catenin (S552)] or for (D) Wnt-targeted proteins (P-GSK3β, cyclin D1, CD44, c-myc, survivin). GAPDH and β-actin were used as reference proteins. (E) Cells were exposed to J1-001-2 for 3–48 h, and blots were probed for LC3-A/B, P-catenin(S552), caspase 3 and GAPDH. (F) Cells were exposed to different concentrations of J1-001-2 for 16 h, and blots were probed for LC3-A/B, caspase 3 and β-actin.