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. 2019 Oct 1;2(6):414–428. doi: 10.1021/acsptsci.9b00049

Figure 4.

Figure 4

Phosphorylation of Akt, JNK, and p38 MAP kinases in human primary PBMC. (A) Time-courses with HU308 (1 μM) and vehicle control. For p-Akt and p-p38 time-points 10–30 min are significantly different from time-matched vehicle controls (p < 0.0001–0.0002; two-way RM ANOVA with Holm-Šídák posthoc test). For p-JNK, all points are indistinguishable from 0 min (p = 0.988; one-way ANOVA). The graph shows mean ± SEM of three independent experiments performed in technical triplicate, each with cells from a separate subject (three subjects in total). (B) Responses to 20 min 1 μM HU308 after pretreatment with PTX, gallein, or vehicle control. Data are normalized and statistically compared to the corresponding vehicle plus HU308 control by two-way RM ANOVA with Holm-Šídák posthoc test. The graph shows independent experiment means (from technical triplicate) as well as overall mean ± SEM of these three independent experiments each performed with cells from a separate subject (three subjects in total).