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. 2020 Jan 23;3(1):148–160. doi: 10.1021/acsptsci.9b00085

Figure 4.

Figure 4

High MYCN expression sensitizes neuroblastoma cells to the combination of pan-AKT inhibitor with conventional cytotoxic drugs. (A) Histogram representation of change in IC50 values in two different neuroblastoma cell lines when chemotherapy agents are used in combination with subtoxic concentrations of AKT inhibitors (IC10) A-674563, CCT128930, or perifosine. (B) Histogram representation of change in IC50 values in a panel of neuroblastoma cells when chemotherapy agents are used in combination with subtoxic concentrations of perifosine (IC10). (C) Dot plot representation of the combination indexes (CI) of chemotherapeutic agents combined to IC10 perifosine in MYCN-amplified neuroblastoma cells. (D) Histogram representation of change in IC50 values in three neuroblastoma cells with MYCN expression modulation when chemotherapy agents are used in combination with subtoxic concentrations of perifosine (IC10). Columns are the means of at least four individual experiments; bars are the SEM. Statistical analysis was performed by comparing the IC50 values of chemotherapy alone or in combination with perifosine using Student’s t-test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).