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C-terminal interactions between PAR2 and SLIGKV. Concentration-response curves of calcium signaling by (a) SLIGKV and (b) SLIGEV at WT (blue) and mutant receptors E232A (green) and E232R/N222Q (magenta). Data are presented as mean ± s.e.m. of n = 3 independent experiments, and numerical values are shown in Table 5. (c) Fold change in potency calculated compared to peptide response at WT.
