Table 2. Potency Data Probing N-Terminal Interactions of Peptide Agonists and PAR2 Receptor with Relevant Single Point Mutations^a.

	SLIGKV	ALIGKV	N-(3-OH-2-Me-propanoyl)-LIGKV
WT	6.06 ± 0.03	5.10 ± 0.03	4.45 ± 0.03
Y82F1.39	5.39 ± 0.03	4.02 ± 0.08	3.57 ± 0.02
D228AECL2	3.96 ± 0.04	<3.5	<3.5
D228NECL2	<3.5	<3.5	<3.5

^apEC₅₀ values, measured by calcium mobilization assays, are presented as mean ± s.e.m. (n = 3 independent experiments).