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. 2019 Mar 18;2(3):183–197. doi: 10.1021/acsptsci.9b00010

Figure 3.

Figure 3

Alanine mutation of ECL2 and ECL3 of AMY3R alters cAMP functional affinity (log KA) in a peptide-specific manner. Functional affinities derived from operational fitting of concentration–response curves in cAMP accumulation for alanine mutation of ECL2 (A-E) and ECL3 (F-J) are displayed as log KA. Significance of changes was established by comparison of the WT to the other receptor mutants following one-way ANOVA and Dunnett’s post-test with P < 0.05 accepted as significant. Mutants that gave significant reductions between 3- and 10-fold are colored orange, and those with reductions greater than 10-fold are colored red. Mutants giving significant increases in log KA are colored green. Where data were insufficiently robust to derive a reliable value for log KA no symbol is shown (ND). Quantitative data are reported in Table 2.