Table 2. Effect of Single Alanine Mutation in AMY₃R ECL2 or ECL3 on cAMP Functional Affinity (log K_A) of Peptides^a.

^aFor each receptor mutant and ligand, concentration–response data for each pathway were fit with the Black and Leff operational model to derive an affinity-independent measure of efficacy and functional affinity (log K_A). Mean, S.E.M., and the individual experimental “n” values are reported. Significance of changes in log K_A of each ligand was determined by comparison of mutant receptors to WT values by a one-way ANOVA and Dunnett’s post-test (p < 0.05 denoted by bold coloured entries. Orange, significant decrease ≤ 10-fold; red, significant decrease > 10-fold; Light green, significant increase ≤ 10-fold; Dark green, significant increase > 10-fold). ND, data were not able to be reliably determined.