Table 5. Effect of Single Alanine Mutation in AMY₃R ECL2 or ECL3 on pERK Signaling Efficacy (log τ_c) of Peptides^a.

^aFor each receptor mutant and ligand, concentration–response data for each pathway were fit with the Black and Leff operational model to derive an affinity-independent measure of efficacy (log τ) and functional affinity. These data were corrected for changes in cell surface expression from FACS to yield log τ_c. Mean, S.E.M., and the individual experimental “n” values are reported. Significance of changes in log τ_c of each ligand was determined by comparison of mutant receptors to WT values by a one-way ANOVA and Dunnett’s post-test (p < 0.05 denoted by bold coloured entries. Red, significant decrease > 10-fold). ND, data were not able to be reliably determined.