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. 2019 Aug 2;2(6):387–401. doi: 10.1021/acsptsci.9b00041

Figure 7.

Figure 7

Effect of αVβ3 targeting compounds on VEGF-induced angiogenesis ex vivo. (A) Mouse aortic rings were stimulated with OptimMEM supplemented with 2.5% FCS and 30 ng/mL VEGF, PBS, or vehicle alone (DMSO). Cilengitide, TDI-4161, or TDI-3761 were added to OptimMEM supplemented with 2.5% FCS and 30 ng/mL VEGF at 1 nM, 10 nM, 100 nM, 1 μM, and 10 μM. The number of sprouts per ring was counted in a blinded fashion using a phase contrast microscope at day 8 postembedding. Data are presented as mean ± SEM. To minimize the potential impact of interanimal variations in angiogenesis, rings from the aortas of four animals were included in each experimental condition. The number of rings included in each experimental condition varied, however, from one to four. To prevent overweighting the impact of any aorta, the number of sprouts from the rings from the same aorta in each experimental condition were averaged, yielding four values for each condition, one for each aorta. The data presented are the mean ± SEMs of these four values. Asterisks above the data bars indicate that the results differ significantly (p < 0.05) from that of the DMSO + VEGF sample using two-tailed Student’s t-test without correction for multiple comparisons.