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Visualization of ligand-induced β-arrestin-2 recruitment to GHSR1a receptor mutants. Live human embryonic kidney (HEK-293)T cells stably expressing β-arrestin-2–green fluorescent protein (GFP) and transiently transfected with GHSR1a: (a) wild-type, (b) L149G (LG), (c) P148A (PA), (d) D353EAA (DEAA), and (e) T360EAA (TEAA) variants were treated with 1 μM L585 and imaged by confocal microscopy at the indicated times (columns).
