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Anti-TGEV-SN-specific IgA level in intestinal lavage after intragastric immunization. Mice received three consecutive doses of 109 CFU/ml LNZ9000-rTGEV-SN, 3 times within a 2-week period. Control mice received 109 CFU/ml pNZ8112/NZ9000, while the negative group received PBS. Intestinal lavages were collected on days 18, 32, 38, and 46 after the first immunization, and were analyzed via ELISA using TGEV as the coating antigen. Bars represent the IgA titer ± S.E.M in each group
