Table 1.

Target, primer/probe sequences and concentrations, and amplification conditions for endpoint PCR and qPCR assays used in this study

PCR/qPCR assay	Target gene	Size of amplicons	Primers/probes sequence	Primers/probes concentrations	Cycling parameters	Reference
Sketa22	ITS-2	77 bp	F, GGT TTC CGC AGC TGG G R, CCG AGC CGT CCT GGT CTA P, FAM-AGT CGC AGG CGG CCA CCG T-TAMRA	300 nM 300 nM 400 nM	10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 45 s at 63 °C	Haugland et al. 2005
HAdVs	Hexon	132 bp	F, GCC ACG GTG GGG TTT CTA AAC TT R: GCC CCA GTG GTC TTA CAT GCA CAT C	250 nM 250 nM	10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C, 20 s at 60 °C, and 20 s at 95 °C	Heim et al. 2003
HPyVs	Homologous T antigen	176 bp	F, AGT CTT TAG GGT CTT CTA CCT TT R, GGT GCC AAC CTA TGG AAC AG P, FAM-AGT CGC AGG CGG CCA CCG T-MGBNFQ	500 nM 500 nM 400 nM	10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C, 15 s at 55 °C, and 60 s at 60 °C	McQuaig et al. 2009