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. 2016 Aug 13;227(9):327. doi: 10.1007/s11270-016-3026-5

Table 1.

Target, primer/probe sequences and concentrations, and amplification conditions for endpoint PCR and qPCR assays used in this study

PCR/qPCR assay Target gene Size of amplicons Primers/probes sequence Primers/probes
concentrations
Cycling parameters Reference
Sketa22 ITS-2 77 bp

F, GGT TTC CGC AGC TGG G

R, CCG AGC CGT CCT GGT CTA

P, FAM-AGT CGC AGG CGG CCA CCG T-TAMRA

300 nM

300 nM

400 nM

10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 45 s at 63 °C Haugland et al. 2005
HAdVs Hexon 132 bp

F, GCC ACG GTG GGG TTT CTA AAC TT

R: GCC CCA GTG GTC TTA CAT GCA CAT C

250 nM

250 nM

10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C, 20 s at 60 °C, and 20 s at 95 °C Heim et al. 2003
HPyVs Homologous T antigen 176 bp

F, AGT CTT TAG GGT CTT CTA CCT TT

R, GGT GCC AAC CTA TGG AAC AG

P, FAM-AGT CGC AGG CGG CCA CCG T-MGBNFQ

500 nM

500 nM

400 nM

10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C, 15 s at 55 °C, and 60 s at 60 °C McQuaig et al. 2009