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. 2016 Jul 28;52(6):877–882. doi: 10.1007/s11262-016-1374-2

Fig. 1.

Fig. 1

Cloning, expression, and purification of recombinant poIFN-λ3 in R. gami B. a Molecular cloning of poIFN-λ3. b SDS-PAGE analysis of samples. M, molecular weight marker; lane 1 the supernatant of bacterial cells harboring pET-poIFN-λ3 without IPTG induced; lane 2 the precipitation of bacterial cells harboring pET-poIFN-λ3 without IPTG induced; lane 3 the supernatant of bacterial cells harboring pET-poIFN-λ3 with 0.4 mM IPTG induced for 16 h at 20 °C; lane 4 the precipitation of bacterial cells harboring pET-poIFN-λ3 with 0.4 mM IPTG induced for 16 h at 20 °C. c Western blot using an anti-His antibody. M, molecular weight marker; lane 1 R. gami B extract transformed by pET-32a (+) (negative control); lane 2 R. gami B extract transformed by pET-poIFN-λ3