Fig. 2.

Amino acid sequences analysis, phylogenetic tree, and prediction of a signal peptide for poIFN-λ3. a Alignment of amino acid sequences for porcine, B. taurus, O. aries, T. belangeri, Pan troglodytes, Equus caballus, Mus musculus, Gallus silurana, and Homo sapiens IFN-λ3. The numbers indicate the amino acid position. Predicted signal peptide: amino acids 1–23 (boxed in blue). Putative N-glycosylation site: amino acids 71–74 (boxed in red). b A phylogenetic tree of the nine identified or predicted IFN-λ3 from different species. The uprooted tree was built using the neighbor-joining method based on the alignment of IFN-λ3 amino acid sequences. The scale bar is 0.1. c The signal peptide of poIFN-λ3 was predicted by SignalP3.0 Server. C, S, and Y scores indicate cleavage sites, ‘signal peptide-ness,’ and combined cleavage site predictions, respectively (Color figure online)