Fig. 3.

Effect of poIFN-λ3 on PEDV infection of Vero E6 cells. a Effect of poIFN-λ3 on PEDV infection and replication under three different conditions. Vero E6 cells were pretreated with poIFN-λ3 (100 ng/mL) for 24 h, and then infected with PEDV CV777 strain (Before) or coincubated with poIFN-λ3 (100 ng/mL) and PEDV CV777 strain at the same time (during), or 12 h after PEDV infection, Vero E6 cells were treated with 100 ng/mL poIFN-λ3 (After). b Time-dependent effect of poIFN-λ3 on PEDV infection. Vero E6 cells were infected with PEDV CV777 strain for 12 h and then treated with poIFN-λ3 at a concentration of 100 ng/mL. The PEDV titer in the supernatant at the indicated time point was titrated by TCID₅₀. c Dose-dependent effect of poIFN-λ3 on PEDV infection. Vero E6 cells were infected with PEDV CV777 strain for 12 h and then treated with poIFN-λ3 at the indicated concentrations. The PEDV titer in the supernatant was analyzed at 48 h postinfection. d, e Real-time quantitative RT-PCR detection of PEDV-N mRNA transcripts relative to β-actin transcripts in the same sample. The mean of three repeat experiments performed in triplicate is shown and error bars represent the SD. d is under three different conditions and e is at different doses of poIFN-λ3