Fig. 2.

Impact of LV-delivered shRNAs on secreted HBV antigens. HepG2.2.15 cells were transduced with LV-HBV-shRNA1 (targeting the RT region), LV-HBV-shRNA2 (targeting the CP region), or LV-HBV-shRNA3 (targeting the DR1 region). At the indicated timepoints post-transduction the levels of HBsAg (a) and HBeAg (b) were determined by ELISA. Untreated HepG2.2.15 cells served as reference, cells transduced with LV-HBV-shRNA-NC expressing an irrelevant shRNA as control for nonspecific effects. Values obtained for the transduced cells were normalized to those obtained for untreated HepG2.2.15 cells which were set as 100 %. All data are shown as mean ± SD from three independent experiments