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Impact of LV-delivered shRNAs on HBV replicative DNA intermediates in intracellular nucleocapsids. HepG2.2.15 cells were transduced with the indicated LV-HBV-shRNA vectors as described in Fig. 4. At 96 h post-transduction, nucleocapsid-associated viral DNAs were isolated and analyzed by Southern blotting, using a 32P-labeled full-length HBV DNA probe. The positions of relaxed-circular (RC) and double-stranded linear (dsL) DNA are indicated
