Fig. 7.

Regulation of HBsAg and HBeAg secretion by DOX-inducible shRNA targeting the DR1 region of HBV. HepG2.2.15 cells were transduced with LV-HBV-shRNA3 alone, or co-transduced with LV-tTR-KRAB; cells were maintained in the absence DOX or in the presence of 5 μg/ml DOX as indicated. Non-transduced HepG2.2.15 cells served as reference. Cell culture supernatants were analyzed at the indicated timepoints for HBsAg (a) and HBeAg (b) by ELISA. Note the absence of suppression in LV-tTR-KRAB co-transduced cells in the absence of DOX, and full restauration of suppression in the presence of DOX. All data shown are the mean from three independent experiments. Error bars indicate SD