Fig. 5.

Measurement of ferritin secretion in the presence of HEV-macro domain. Huh-7 cells transfected by either empty vector or pcDNA-macro clone plasmid. Quantitation of ferritin was done by Enzyme Linked Immunosorbent Assay. Cells were either kept untreated or treated with 100 µM Ferric Ammonium Citrate (FAC) or 100 µM Pyridoxal Isonicotinoyl Hydrazone (PIH) for 24 h. In some experiments, cells were treated with Brefeldin-A (1 µg/ml) for 3 h. Values correspond to triplicate samples (mean ± SD, *p ≤ 0.05; **p ≤ 0.005)