Fig. 6.

Measurement of ferritin secretion in the presence of subgenomic replicon of HEV. a Replication kinetics of HEV. Huh-7 cells were either left untransfected (mock) or co-transfected with capped RNA transcripts of pSK-E2-Luc and pGL4.10. At different days post transfection, cells were harvested and luciferase activity was measured. b Measurement of the secretion of intacellular ferritin in the presence of HEV replicon. Ferritin ELISA of the cell culture supernatant was performed at different time intervals. Values correspond to triplicate samples (mean ± SD)