Fig. 1.

Proteasome inhibition reduces IBDV release. (A) DF-1 cells were infected with IBDV (MOI = 20) at 39°C for 1 h, washed with PBS, and incubated with different concentrations of proteasome inhibitors, MG132 and lactacystin, for 6 h, and washed with PBS, and then incubated with 10% DMEM medium without proteasome inhibitors. Viral titers in the supernatants of IBDV-infected DF-1 cells were determined after 21 h p.i. by TCID₅₀ assay. (B) DF-1 cells were infected with different amounts of IBDV and then treated with 20 μM of MG132 for 6 h, and continued to incubate at 39°C. Virus titers of supernatants collected at 21 h p.i. as determined by TCID₅₀ assay are shown. Values are means ± standard errors (SE) of three independent experiments