Table 1.

Primer sequences along with expected PCR product size and the thermal cycling condition

Primer name	Primer sequence		Product size (bp)	Reference
S1HS	(5′)-CTATACCCAATGGTAGGA-(3′)		885	Brandao et al. (2003)
S1HA	(5′)-CTGAAACACGACCGCTAT-(3′)
S1N	(5′)-GTTTCTGTTAGCAGGTTTAA-(3′)		488
S1NA	(5′)-ATATTACACCTATCCCCTTG-(3′)
Thermal cycling condition
	Pre-denaturation	Denaturation	Primer annealing	Primer extension	Final extension
Outer primers	94 °C; 180 s	94 °C; 60 s	53.4 °C; 90 s	72 °C; 60 s	72 °C; 600 s
		×35 cycles
Internal primers	94 °C; 180 s	94 °C; 60 s	58.4 °C; 90 s	72 °C; 60 s	72 °C; 600 s
		×25 cycles