Table 1.
Primers used for amplification of the full-length genome of the CDV-TM-CC strain
| Primer | Sequence (5′ → 3′) | Position |
|---|---|---|
| A1F | ACCAGAAAAAGTTGGCTATG | 1 |
| AIR | GCFGTTFCACCCATCFGTTG | 1036 |
| A2F | GAACAAGCCTAGAAFTGCTG | 842 |
| A2R | TGAGGGCTTTGAGGCATTCC | 1858 |
| A3F | FTCAAGACCAGFGFTACAFC | 1683 |
| A3R | GCAGGTFGGCGGACATFCTC | 2627 |
| A4F | TCTGCAGTTCCCACGCAATC | 2427 |
| A4R | CCTGCAGTTCGCCTTACCAC | 3748 |
| A5F | GFGAFAGAFCCAGGACTCGG | 3540 |
| A5R | CTCACCTAATTCFGCFFTGG | 5495 |
| A6F | FAFGGTGCATFGGAATAGCC | 5296 |
| A6R | CCTACATCTAACCTCTCAAG | 6649 |
| AT | GTGTAAGTGTTATAGCACAG | 6452 |
| A7R | AAGGGTTCCCATGACGTTFG | 8271 |
| ASF | TGGATCAAGTTGAAGAGGTG | 8061 |
| ASR | CGTGCATACTCTAAAATAGC | 9127 |
| A9F | GGACTTAGGTATGATGACTG | 8928 |
| A9R | CCFGAGAGAGAAGGFGATGT | 9932 |
| A10F | FTAAFGTGTTAGTGTCFCGG | 9631 |
| A10R | CAFFFFAGCGAATAGCCTCC | 10676 |
| A11F | ATTCCTCAGGTACAACCCAC | 10478 |
| A11R | GGTCAAACCCTTTCTCAATG | 11682 |
| A12F | CCACAGAATACTTTGTAGCC | 11428 |
| A12R | AFCGAFGAGCGGAFCAFGCC | 12413 |
| A13F | CTTTAGCCGCTTTCTTGATG | 12160 |
| A13R | ACAGTCTAAATAAGTGCTCG | 13080 |
| A14F | CCGCTCATCGATCAAGACTC | 12402 |
| A14R | CTATTGATATCATCATCGCC | 13438 |
| A15F | GACTTTATAACCAGAGTCAC | 13261 |
| A15R | ACATGCAGFAGAGFTGATCC | 14321 |
| A16F | AFCACTGAFGCFGFTGGATG | 14100 |
| A16R | ACCAGACAAAGCTGGGTATG | 15690 |
| B1F | ACCAGAAAAAGTTGGCT | 1 |
| B1R | TCCGTTGTCTTGGATGCT | 1315 |
| B2F | GCTCTGGAGTTATGCTAT | 1100 |
| B2R | TTAACCTCTTCACCGCTG | 2162 |
| B3F | CCTACCATGTCAGCAAAG | 1817 |
| B3R | AAGCGATAGTGAAAGCAG | 3378 |
| B4F | TACCGCACCTTCCAAAGC | 3168 |
| B4R | GCACTCTAATCTCCATAG | 4331 |
| B5F | CGATGTACTGGTAAGATG | 4212 |
| B5R | ACCTAATTCTGCTFTGGT | 5492 |
| B6F | AATCCAATGCAACCAACT | 5251 |
| B6R | AGCAGGGCCTAAGGCAAC | 6623 |
| B7F | AGTCCTGATAAATTGCTG | 6489 |
| B7R | GACTCGAATCTTFTGCG | 7828 |
| BSF | TGGAGCTACTACTFCA | 7645 |
| B8R | ATACGGACTAAATTCTCAAC | 8874 |
| B9F | AGATGTCCTTACTGAGTC | 8584 |
| B9R | GAGAGAGAAGGTGATGTC | 9929 |
| B10F | ACTTTGCACGTCAGAATC | 9786 |
| B10R | CCTAGCTATACTTFTCAG | 11585 |
| B11F | TTGGCAGCACATGAAAGC | 11298 |
| BUR | GACCCTTCGCCAACTTAG | 12483 |
| B12F | GAGGGAGGCAATTGCTGG | 12242 |
| B12R | TGGGAGTCAAGAGAGGGT | 13705 |
| B13F | GGTCAATGTGCTGCAATC | 13491 |
| B13R | TCTCTCGTTGTCCAGTTC | 14499 |
| B14F | TACGTCTGAAGAACATGG | 14363 |
| B14R | ACCAGACAAAGCTGGGTA | 15690 |
Two sets of forward (F) and reverse (R) primers were used (sets A and B) for verification of the sequence in redundant amplicons