Fig. 3.

Naked-eye inspection of the RT-LAMP reaction stained with SYBR GreenI under normal light. Tubes 1–8, the concentrations of JEV RNA are ranged from 2.4 × 10⁷ to 2.4 copies/μl in serial 10-fold dilutions. Tubes 1–6 are positive amplifications (the original orange color of SYBR GreenI turn to be green in the online version). Tubes 7–8 are negative reactions (the tubes remain orange color in the online version). It demonstrates the detection limit of JEV RT-LAMP is 2.4 × 10² copies/μl by naked-eye inspection