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Comparisons of RT-PCR, real-time PCR and RT-LAMP methods for detecting sensitivity of JEV RNA
| Methods | Concentrations of JEV RNA (copies/μl) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 2.4 × 107 | 2.4 × 106 | 2.4 × 105 | 2.4 × 104 | 2.4 × 103 | 2.4 × 102 | 2.4 × 101 | 2.4 × 100 | ||
| Conventional RT-PCR | + | + | + | + | + | + | − | − | |
| Real-time RT-PCR | + | + | + | + | + | + | + | − | |
| RT-LAMP | |||||||||
| Naked-eye inspection | + | + | + | + | + | + | − | − | |
| Agarose gel analysis | + | + | + | + | + | + | + | − | |
+ Positive reaction; − Negative reaction
