Table 1. Replication of BgV in insects and vertebrates.

Insect and vertebrate cell lines were inoculated in triplicates with BgV and WNV at MOI 1 for 1h 30min, the inoculum removed and the cells washed before incubating the replenished cultures at 28°C or 37°C. The inoculated cell culture supernatants were harvested five days p.i., titrated on C6/36 cells and analysed by fixed-cell ELISA.—represents no replication, + represents a titre over 10² TCID₅₀IU/mL, ++ represents a titre over 10⁴ TCID₅₀IU/mL, +++ represents a titre over 10⁶ TCID₅₀IU/mL. In a few instances, not all replicates were positive, in which case the titre represented is the average of the positive replicates only.

Cell line		Temperature	Replication levels
Animal	Name		BgV	WNVKUN
Mosquito	C6/36*	28°C	+++	+++
	HSU		-	-
	MOS55		+++	++
Drosophila	S2		++	++
Viper	VSW	28°C	+ˠ	++
Crocodile	3CPL		+ˠ	++
Dog	MDCK	37°C	+	++
Pig	PK15		+ˠ	++
	LLCPK1		+	++
	ST		++	+++
Opossum	OK*		++	++
Hamster	BHK*		++	+++
	BSR		+++	+++
Human	HEK293*		+	+++
	SW13		+++	+++
Frog	A6	28°C	-	+
Crocodile	3CPK		-	++
	1LV		-	++
Monkey	Vero*	37°C	-	+++
Chicken	DF-1*		-	+++
Rabbit	RK-13*		-	++
Cow	MDBK		-	-
Mouse	MEFWT		-	+++
	MEFIFNAR-/-		-	+++
	MEFRNL-/-		-	+++
Wallaby	JU56		-	+ˠ

- < limit of detection (10² TCID₅₀IU/mL);

+ > 10² TCID₅₀IU/mL; ++ > 10⁴ TCID₅₀IU/mL; +++ > 10⁶ TCID₅₀IU/mL

ˠ Not all replicates were positive

*BgV host-restriction in these cell lines was published previously in Colmant et al. 2016. The results presented here are from repeat experiments in these cell lines.