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. 2020 Mar 23;15(3):e0230366. doi: 10.1371/journal.pone.0230366

Fig 3. Redox state of C8J_1298 in various strains of C. jejuni 81116.

Fig 3

(A) Strains lacking membrane Dsb proteins: ΔdsbD (AB2), ΔdsbI (AG4) and ΔdsbB (AG3). (B) Strain lacking CjDsbA1 (KBO1). Bacterial cultures were treated with 10% TCA, followed by alkylation with AMS. Cellular proteins including the reduced (red; DTT treated, modified with AMS) and the oxidized (ox; non-modified with AMS) wild type controls were separated by 14% SDS-PAGE under non-reducing conditions, and Western blot analysis using antibodies against C8J_1298 was performed. Each lane contains proteins isolated from the same amount of bacteria. (C) Table and (D) plot presents equlibrium beetwen reduced and oxidized forms of C8J_1298 in various C. jejuni 81116 strains. Proportion of reduced and oxidized forms of protein was estimated using ImageLab™ (Bio-Rad). Result is the average of three independent experiments, with two technical repetitions (n = 3). The figure presents a representative result.