Fig 6. C8J_1298 activity in E. coli dsb deficient strains.

(A) Motility assay: Exponentially growing E. coli strains were spotted on soft agar plates and incubated 24h at 37°C. C8J_1298 restored motility in both dsbA and dsbAdsbB deficient strains Table and plot present diameter of growth on soft agar plates of tested strains. Presented average is the result of three independent experiments (n = 3). (B) Copper sensitivity assay: Exponentially growing E. coli strains were 10-fold diluted and spotted on BHI-agar plates supplemented with arabinose and CuCl₂. Presence of C8J_1298 only slightly increased resistance to copper toxicity in E. coli dsbC lacking strain. (C) Mucoid phenotype assay: E. coli strains were cultivated on M63 minimal medium. C8J_1298 restored mucoid phenotype in mdoG deficient E. coli strain. In all assays three independent experiments were performed (n = 3) and bacterial cultures growing without arabinose supplementation were used as controls. The figures present a representative result.