Fig 7.

Redox state of C8J_1298 in (A) E. coli strains deficient in proteins of oxidative Dsb pathway. (B) E. coli strain deficient in EcDsbC protein Bacterial cultures were treated with 10% TCA, followed by alkylation with AMS. Cellular proteins including the reduced (red; DTT treated, modified with AMS) and the oxidized (ox; non-modified with AMS) wild type controls were separated by 14% SDS-PAGE under non-reducing conditions, and Western blot analysis using antibodies against C8J_1298 was performed. Each lane contains proteins isolated from the same amount of bacteria. (C) Table and (D) plot presents equlibrium beetwen reduced and oxidized forms of C8J_1298 in various E. coli strains. Proportion of reduced and oxidized forms of protein was estimated using ImageLab^™ (Bio-Rad). Result is the average of three independent experiments (n = 3). (*) unspecific serum reaction. The figure presents a representative result.