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. 2020 Mar 23;15(3):e0230366. doi: 10.1371/journal.pone.0230366

Table 1. Strains used in this study.

Name Relevant characteristics Source/Ref.
Escherichia coli STRAINS
TG1 supE44 hsdΔ 5 thi Δ(lacproAB) F’ [traD36 proAB+ lacIq lacZΔM15] [83]
Rosetta(DE3) pLysS F‾ ompT hsdSB(rB‾mB‾) gal dcm (DE3) pLysS RARE (CmR) Novagen
JCB816 MC1000 phoR λ102 [84]
JCB817 MC1000 phoR λ102 dsbA::kan1 [84]
JCB818 MC1000 phoR λ102 dsbA::kan1, dsbB::kan [84]
JFC383 JCB816 dsbC::kan JFC collection
PL260 MC1000 mdoG::kan1 [63]
PL263 MC1000 mdoG::kan1; dsbC::kan2 [63]
PL284 PL263 carrying pBAD33 KmR CmR [63]
PL285 PL263 carrying pJFC355 (dsbC+ in pBAD33) KmR CmR [63]
BL21/EcdsbA BL21 carrying EcdsbA+ in pET28a KmR JFC collection
BL21/EcdsbC BL21 carrying EcdsbC+ in pET28a KmR JFC collection
KBO1436 Rosetta(DE3) pLysS carrying pUWM1430 (CjdsbA1+ in pET28a) KmR CmR this study
KBO1441 Rosetta(DE3) pLysS carrying pUWM1432 (CjdsbA2+ in pET24a) KmR CmR this study
AB1463 Rosetta(DE3) pLysS carrying pUWM1462 (c8j_1298+ in pET24a) KmR CmR this study
AB1518 (ΔdsbA/pl1298) JCB817 carrying pUWM1514 (ss’pelB-c8j_1298+ in pMPMA6) KmR ApR this study
AB1519 (ΔdsbC/pl1298) JCF383 carrying pUWM1514 (ss’pelB-c8j_1298+ in pMPMA6) KmR ApR this study
AB1520 (ΔmdoGΔdsbC/pl1298) PL263 carrying pUWM1514 (ss’pelB-c8j_1298+ in pMPMA6) ApR KmR this study
AB1524 (ΔdsbAΔdsbB/pl1298) JCB818 carrying pUWM1514 (ss’pelB-c8j_1298+ in pMPMA6) ApR KmR this study
Campylobacter jejuni 81116 STRAINS
wild type—81116 (NCTC11828) parental strain [33]
ΔdsbA1[old] (AG1) CjdsbA1::kan [39]
ΔdsbA1[new] (KBO1) CjdsbA1::cat this study
ΔdsbA1ΔdsbA2 (KBO2) CjdsbA1::kan, dsbA2::cat this study
ΔdsbA2 (AG2) CjdsbA2::cat [39]
ΔdsbB (AG3) CjdsbB::kan [39]
ΔdsbI (AG4) CjdsbI::cat [39]
Δc8j1298 (AB1) c8j_1298::kan this study
ΔdsbD (AB2) CjdsbD::cat this study
Δc8j1298ΔdsbD (AB3) c8j_1298:kan; CjdsbD::spec this study
ΔdsbA1Δ1298 (AB4) CjdsbA1::cat; c8j_1298::kan this study
ΔdsbA1/chrA1 [old] (AG1/ chrA1) CjdsbA1::kan/ chrA1 (chromosomal complementation CjdsbA1 performed using pUWM1526) KmR SpecR this study
ΔdsbA1/chrA1 [new] (KBO1/chrA1) CjdsbA1::cat/ chrA1 (chromosomal complementation CjdsbA1 performed using pUWM1526) CmR SpecR this study
ΔdsbA1ΔdsbA2/chrA2 (KBO2/chrA2) CjdsbA1::kan, dsbA2::cat/ chrA2 (chromosomal complementation CjdsbA2 performed using pUWM1516) KmR CmR SpecR this study
Δc8j1298/chr1298 (AB1/chr1298) c8j_1298:kan/ chrc8j_1298 (chromosomal complementation c8j_1298 performed using pUWM1513) KmR CmR this study
Δc8j1298/pl1298 (AB1/p1501) C8J_1298:kan/ pUWM1501 (plasmid complementation c8j_1298) KmR CmR this study
Δc8j1298ΔdsbA1/chrA1 (AB4/chrA1) CjdsbA1::cat; c8j_1298::kan/ chrA1 (chromosomal complementation CjdsbA1 performed using pUWM1526) KmR CmR SpecR this study
Δc8j1298ΔdsbA1/chr1298 (AB4/chr1298) CjdsbA1::cat; c8j_1298::kan/ chr1298 (chromosomal complementation c8j_1298 performed using pUWM1517) KmR CmR SpecR this study