| General cloning and expression vectors |
| pJet 1.2 blunt |
ApR, CloneJET PCR Cloning Kit |
ThermoFisher Scientific |
| pBluescript KS/SK |
ApR; LacZα |
Stratagen |
| pET22b |
ApR; ss’pelB, IPTG inducible |
Novagen |
| pET24a |
KmR, IPTG inducible |
Novagen |
| pET28a |
KmR, IPTG inducible |
Novagen |
| pNBSpec |
SpecR, source of spec cassette |
[85] |
| pBF14 |
KmR, source of aph cassette |
J. van Putten |
| pMPMA6 |
ApR, SpecR, ParaBAD
|
[60] |
| pRY109 |
CmR, source of cat cassette |
[52] |
| pRY111 |
CmR, shuttle vector E. coli–C. jejuni
|
[52] |
| pBC49 |
CmR, vector for chromosomal complementation vector by double crossing-over in c8j_0049 pseudogene |
this study |
| pBS49 |
SpecR, vector for chromosomal complementation vector by double crossing-over in c8j_0049 pseudogene |
this study |
| Plasmid for complementation experiments–final vectors |
| pUWM1513 |
c8j_1298 in pBC49 –suicide vector for chromosomal complementation in C. jejuni c8j_0049 pseudogene, CmR
|
this study |
| pUWM1517 |
c8j_1298 in pBS49 –suicide vector for chromosomal complementation in C. jejuni c8j_0049 pseudogene, SpecR
|
this study |
| pUWM1526 |
CjdsbA1 in pBS49 –vector for chromosomal complementation in C. jejuni c8j_0049 pseudogene, SpecR
|
this study |
| pUWM1516 |
CjdsbA2 in pBS49 –suicide vector for chromosomal complementation in C. jejuni c8j_0049 pseudogene, SpecR
|
this study |
| pUWM1501 |
c8j_1298 in pRY111 –shuttle vector for plasmid complementation in C. jejuni, CmR
|
this study |
| pUWM1514 |
ss’pelB-c8j_1298 in pMPMA6 –vector for plasmid complementation in E. coli, inducible |
this study |
| Plasmid for complementation experiments–intermediate constructs |
| pUWM1500 |
c8j_1298 in pJet 1.2 blunt |
this study |
| pUWM1506 |
CjdsbA1 in pJet 1.2 blunt |
this study |
| pUWM1505 |
CjdsbA2 in pJet 1.2 blunt |
this study |
| pUWM1485 |
c8j_1298 in pET22b |
this study |
| Plasmids for allel-exchange mutagenesis |
| pUWM1306 |
CjdsbA1::aph in pGEM-T Easy |
[39] |
| pUWM1458 |
CjdsbA1::cat in pGEM-T Easy |
this study |
| pUWM1483 |
c8j_1298::aph in pBluescript II KS |
this study |
| pUWM1503 |
CjDsbD::spec in pBluescript II KS |
this study |
| pUWM1512 |
CjDsbD::cat in pBluescript II KS |
this study |
| Plasmids for recombinant Dsb overexpression and purification |
| pUWM1430 |
CjdsbA1-His6 in pET28a |
this study |
| pUWM1432 |
CjdsbA2-His6 in pET24a |
this study |
| pUWM1462 |
c8j_1298-His6 in pET24a |
this study |
| pET28a/EcdsbA
|
EcdsbA in pET28a |
JFC collection |
| pET28a/EcdsbC
|
EcdsbC in pET28a |
JFC collection |
