Fig. 3.

RING domain was not required for restriction of PRRSV. a Full-length and serial truncations of TRIM22 with deletion (∆) of various domains. Numbers indicate the residues where deletions begin or end. MARC-145 cells transfected with Flag–TRIM22 (WT or domain lacking mutants) were infected with 0.5 MOI of PRRSV for 48 h. b Production of progeny virus were determined using TCID₅₀ assay. c The total cellular RNA was extracted and the mRNA levels of PRRSV N gene were determined by qPCR