Fig. 6.

The regions responsible for the interaction of TRIM22 with N protein. a The schematic diagram of N protein individual mutants investigated in this study. b The interaction of TRIM22 with N protein mutants by Co-IP. HEK293T cells were co-transfected with the indicated plasmids. The cell lysates were immunoprecipitated with an anti-HA mAb and further probed with an anti-HA or anti-Flag antibody. c The interaction of N protein with TRIM22 deletion mutants by Co-IP. HEK293T cells were co-transfected with the indicated plasmids. The cell lysates were immunoprecipitated with an anti-Flag mAb and further probed with an anti-HA or anti-Flag polyclonal antibody